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Zygmunt Gryczynski

Zygmunt Gryczynski

W.A. "Tex" Moncrief, Jr. Chair of Physics

817-257-4209 SWR 182

Program Affiliations

Davis CollegePhysics & Astronomy

Education

MS, University of Gdansk, 1982
Ph.D., University of Gdansk, 1987

Areas of Focus

  •  Spectroscopy and Fluorescence: Absorption; Linear Dichroism; Fluorescence; Fluorescence Polarization; Phosphorescence
  • Probe Development. Novel fluorophores for molecular and cellular imaging: Organic fluorophores; Quantum dots; Nanoparticles
  • Time-Resolved Spectroscopy: Time-resolved fluorescence and phosphorescence; Excited state processes/dynamics.
  • Forster Resonance Energy Transfer (FRET): Steady-state and time-resolved applications at molecular and cellular levels.
  • Molecular/Macro-Molecular Dynamics: Protein Dynamics; Protein Dissociation/Associatio; DNA/RNA.
    Cooperative Binding: Oxygen binding/transport by hemoglobin; Ligand binding; Macromolecular binding.
  • Biomarkers – Enhanced Fluorescence Based Detection. Cardiac Risk Assessment and Cancer Detection.
    Imaging: Tissue; Cell; Single molecule.
  • Fluorescence Based Sensing: Lifetime based sensing; Novel sensing; Plasmons enhanced sensing.
    Multi-Photon Processes: Multi-Photon Induced Fluorecence (Two-Photon, Three-Photon, Four-Photon Excitation); Light Quenching by Stimulated Emission; Two-Pulse Fluorescence.
  • Total Internal Reflection Fluorescence: Steady-state; Time-resolved.
  • Metal Enhanced Fluorescence (MEF): Metallic nanoparticles/nanostructures enhanced detection; Localized surface plasmons.
  • Surface Plasmons Enhanced Detection (SPED): Surface plasmon enhanced fluorescence (SPCE); Surface plasmon resonance (SPR) stimulated emission.
  • Optical and Quantum Processes to Study Biological System in Nanoscale: Proteins, DNA/RNA, Cells, Tissue
  • Gryczynski, Z., Jablonska, A., Lee, B., Laursen, B. W., Sørensen, T. J., Pham, D., Sagoo, R., Pham, T., and Gryczynski, I. (2026). Room temperature luminescence of a triangulenium dye ADOTA in PVA films. Methods and Applications in Fluorescence. https://iopscience.iop.org/article/10.1088/2050-6120/ae484d
  • Grubbs, M. A., Lee, B., Ojeda-Hernandez, L., Dzyuba, S. V., Gryczynski, Z., and Coffer, J. L. (2026). Exceptional fine structure in the photoluminescence excitation spectra of perovskites: Evidence for direct exciton phonon coupling. Applied Physics Letters, 128(8):082104. https://pubs.aip.org/aip/apl/article/128/8/082104/3381318
  • Jablonska, A., Lee, B., Petty, R. M., Pham, D., Sagoo, R., Pham, T. T., Gryczynski, Z., and Gryczynski, I. (2026). Room-Temperature Phosphorescence of Quinine Sulfate in PVA Films: The Effect of Humidity. Optics, 7(1):7. https://www.mdpi.com/2673-3269/7/1/7
  • Ta, D. D., Lee, B., Jablonska, A., Burnett, W. L., Sagoo, R., Pham, D., Ceresa, L., Chavez, J. L., et al. (2025). Symmetric Bis-Kryptopyrrol-2-Yl Squaraine as a Viscosity Probe and Dual-State Emitter. ChemPhotoChem. https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cptc.202500024
  • Petty, R. M., Ceresa, L., Alexander, E., Pham, D., Sabnis, N., Fudala, R., Lacko, A. G., Krishnamoorthy, R. R., Gryczynski, Z., and Gryczynski, I. (2025). Fluorescence Resonance Energy Transfer for Drug Loading Assessment in Reconstituted High-Density Lipoprotein Nanoparticles. International Journal of Molecular Sciences, 26(7):3276. https://www.mdpi.com/1422-0067/26/7/3276
  • Lee, B., Gryczynski, I., and Gryczynski, Z. (2025). Demonstration of Intermolecular Triplet-Singlet FRET in Dye-Doped PVA Films at Room Temperature. The Journal of Physical Chemistry A. https://pubs.acs.org/doi/full/10.1021/acs.jpca.5c00096
  • Ponomarchuk, O., Boudreault, F., Gryczynski, I., Lee, B., Dzyuba, S. V., Fudala, R., Gryczynski, Z., et al. (2025). Nanoscale Viscometry Reveals an Inherent Mucus Defect in Cystic Fibrosis. ACS Nano. https://pubs.acs.org/doi/10.1021/acsnano.4c14927
  • Lee, B., Jablonska, A., Pham, D., Sagoo, R., Gryczynski, Z., Pham, T. T., and Gryczynski, I. (2025). Luminescence Properties of Hoechst 33258 in Polyvinyl Alcohol Films. International Journal of Molecular Sciences, 26(2):514. https://www.mdpi.com/1422-0067/26/2/514
  • lusarczyk, L., Rzd, K., Niedzielski, G., Gurba, M., Chavez, J., Ceresa, L., Kimball, J., Gryczyski, I., Gryczyski, Z., et al. (2024). Understanding the synergistic interaction between a 1,3,4-thiadiazole derivative and amphotericin B on Candida cell membranes. Scientific Reports. https://www.nature.com/articles/s41598-024-83180-2
  • Lee, B., Alexander, E., Pham, D., Gago, M., Matwijczuk, A., Gryczynski, Z., and Gryczynski, I. (2024). Spectral properties of 4-methylumbelliferone in PVA films; long-lived room temperature phosphorescence. Methods and Applications in Fluorescence. https://iopscience.iop.org/article/10.1088/2050-6120/ad9885
  • Czarnomska, M., Lewkowicz, A., Gruszczyska, E., Walczewska-Szewc, K., Gryczyski, Z., Bojarski, P., and Steinborn, S. (2024). Preliminary Investigation of a Potential Optical Biosensor Using the Diamond Nucleic Acid Dye. Biosensors, 14(11):546. https://www.mdpi.com/2079-6374/14/11/546
  • Alexander, E., Ceresa, L., Pham, D., Gryczynski, Z., and Gryczynski, I. (2024). Effect of Annealing on the Room Temperature Luminescence of Coumarin 106 in PVA Films. Methods and Applications in Fluorescence. https://iopscience.iop.org/article/10.1088/2050-6120/ad06dc
  • Ceresa, L., Chavez, J., Bus, M. M., Budowle, B., Kitchner, E., Kimball, J., Gryczynski, I., and Gryczynski, Z. (2023). Multi intercalators FRET enhanced detection of minute amounts of DNA. European Biophysics Journal. https://link.springer.com/article/10.1007/s00249-023-01655-y
  • Chavez, J., Ceresa, L., Kitchner, E., Pham, D., Gryczynski, Z., and Gryczynski, I. (2023). Room Temperature Phosphorescence of 5,6-Benzoquinoline. Methods and Applications in Fluorescence. https://iopscience.iop.org/article/10.1088/2050-6120/acc716
  • Chavez, J., Ceresa, L., Kitchner, E., Pham, D., Gryczynski, Z., and Gryczynski, I. (2023). Room temperature phosphorescence of 2-aminopyridine with direct triplet state excitation. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. https://www.sciencedirect.com/science/article/abs/pii/S1386142523003256
  • Kitchner, E., Seung, M., Chavez, J., Ceresa, L., Kimball, J., Gryczynski, I., and Gryczynski, Z. (2022). Fluorescence measurements: importance of G-factor correction, magic angle, and observation wavelength. Methods and Applications in Fluorescence. https://iopscience.iop.org/article/10.1088/2050-6120/ac92c5
  • Ceresa, L., Chavez, J., Bus, M., Budowle, B., Kitchner, E., Kimball, J., Gryczynski, I., and Gryczynski, Z. (2022). Förster Resonance Energy Transfer-Enhanced Detection of Minute Amounts of DNA. Analytical Chemistry, 94(12):5062-5068. https://pubs.acs.org/doi/10.1021/acs.analchem.1c05275
  • Chavez, J., Ceresa, L., Reeks, J. M., Strzhemechny, Y., Kimball, J., Kitchner, E., Gryczynski, Z., and Gryczynski, I. (2022). Direct Excitation of Tryptophan Phosphorescence. A New Method for Triplet States Investigation. Methods and Applications in Fluorescence, 10(2). https://iopscience.iop.org/article/10.1088/2050-6120/ac4c9a
  • Olejniczak, A., Rich, R. M., Gryczynski, Z., and Cichy, B. (2022). Non-excitonic defect-assisted radiative transitions are responsible for new D-type blinking in ternary quantum dots. Nanoscale Horizons, 7(1):63-76. https://pubs.rsc.org/en/content/articlelanding/2021/nh/d1nh00424g
  • Rice, Q., Raut, S., Burney, K., Gryczynski, Z., Gryczynski, I., Yu, W. W., Tabibi, B., and Seo, J. (2021). Time-Resolved and Temperature-Dependent Fractional Amplitude Contributions to the Broadband Emission of CdSe Quantum Dots. Crystals, 11(11):1284. https://www.mdpi.com/2073-4352/11/11/1284
  • Ceresa, L., Kitchner, E., Chavez, J., Seung, M., Bus, M., Budowle, B., Gryczynski, I., and Gryczynski, Z. (2021). A novel approach to imaging and visualization of minute amounts of DNA in small volume samples. Analyst, 146(21):6520-6527. https://pubs.rsc.org/en/content/articlelanding/2021/an/d1an01391b
  • Leonce, B. L., Harvey, O., Gryczynski, Z., Hockaday, W. C., and Adegboyega, N. F. (2021). Structure−Energy−Photochemical Activity Relationships in Fluorophoric Water-Extracted Organic Matter from (Un)charred Plant Materials. ACS ES&T Water, 1(4):859-870. https://pubs.acs.org/doi/abs/10.1021/acsestwater.0c00208
  • Ceresa, L., Kimball, J., Chavez, J., Kitchner, E., Nurekeyev, Z., Doan, H., Borejdo, J., Gryczynski, I., and Gryczynski, Z. (2021). On the origin and correction for inner filter effects in fluorescence. Part II: secondary inner filter effect - the proper use of front-face configuration for highly absorbing and scattering samples. Methods and Applications in Fluorescence, 9(3). https://iopscience.iop.org/article/10.1088/2050-6120/ac0243
  • Kitchner, E., Chavez, J., Ceresa, L., Bus, M. M., Budowle, B., and Gryczynski, Z. (2021). A novel approach for visualization and localization of small amounts of DNA on swabs to improve DNA collection and recovery process. Analyst, 146(4):1198-1206. https://pubs.rsc.org/en/content/articlehtml/2021/an/d0an02043e
  • Rice, Q., Raut, S., Chib, R., Gryczynski, Z., Gryczynski, I., Wang, A., Yu, W. W., Tabibi, B., and Seo, J. (2020). Time-resolved and Temperature-dependent Broadband Emission of Plasmon-coupled Quantum Dots. Journal of Materials and Applications, 9:99-106. http://xpublication.com/index.php/jma/article/view/461
  • Chavez, J., Kimball, J., Ceresa, L., Kitchner, E., Shtoyko, T., Fudala, R., Borejdo, J., Gryczynski, Z., and Gryczynski, I. (2021). Luminescence properties of 5-Bromoindole in PVA films at room temperature: Direct triplet state excitation. Journal of Luminescence, 230:117724. https://www.sciencedirect.com/science/article/abs/pii/S0022231320316914
  • Kimball, J., Chavez, J., Ceresa, L., Kitchner, E., Nurekeyev, Z., Doan, H., Szabelski, M., Borejdo, J., Gryczynski, I., and Gryczynski, Z. (2020). On the Origin and Correction for Inner Filter Effects in Fluorescence. Part I: Primary Inner Filter Effect-The Proper Approach for Sample Absorbance Correction. Methods and Applications in Fluorescence, 8(3). https://iopscience.iop.org/article/10.1088/2050-6120/ab947c
  • Chavez, J., Ceresa, L., Kitchner, E., Kimball, J., Shtoyko, T., Fudala, R., Borejdo, J., Gryczynski, Z., and Gryczynski, I. (2020). On the Possibility of Direct Triplet State Excitation of Indole. Journal of Photochemistry and Photobiology B: Biology, 208:111897. https://www.sciencedirect.com/science/article/abs/pii/S101113442030347X
  • Gryczynski, Z., Kimball, J., Fudala, R., Chavez, J. L., Ceresa, L., Szabelski, M., Borejdo, J., and Gryczynski, I. (2020). Photophysical properties of 2-Phenylindole in poly (vinyl alcohol) film at room temperature. Enhanced phosphorescence anisotropy with direct triplet state excitation. Methods and Applications in Fluorescence, 8(1). https://iopscience.iop.org/article/10.1088/2050-6120/ab6366
  • Raut, S., Garud, A., Nagarajan, B., Sabnis, N., Remaley, A., Fudala, R., Gryczynski, I., Gryczynski, Z., Dzyuba, S. V., Borejdo, J., and Lacko, A. G. (2020). Probing the Assembly of HDL Mimetic, Drug Carrying Nanoparticles, using Intrinsic Fluorescence. Journal of Pharmacology and Experimental Therapeutics, 372(3):113-121. https://jpet.aspetjournals.org/content/373/1/113
  • Gryczynski, Z., and Gryczynski, I. (2019). Practical Fluorescence Spectroscopy (book). CRC Press / Taylor & Francis Group. https://www.routledge.com/Practical-Fluorescence-Spectroscopy/Gryczynski-Gryczynski/p/book/9781439821695
  • Photonics Approaches to Ultrasensitive Fluorescence Based Detection in Biomedical Diagnostics and Imaging. 14th International Symposium of Nanomedicine (ISNM 2021). Shimane University, November 17– November 19, 2021. Keynote Speech. 
  • Fluorescence Measurments. Probes and Standards. 17th European Short Course on Principles & Applications of Time-Resolved Fluorescence Spectroscopy. Berlin, Germany, November 8-11, 2021.
  • Analitycal Applications of Fluorescence. 17th European Short Course on Principles & Applications of Time-Resolved Fluorescence Spectroscopy. Berlin, Germany, November 8-11, 2021.
  • From Multi-Photon Absorption and Stimulated Emission to Forbidden S0→T1 Transition. Multiphoton Microscopy in the Biomedical Sciences XX. 2–4 February 2020, Photonics West, SPIE. San Francisco, CA

Last Updated: August 07, 2026

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